All dairy laboratories are evaluated every two years by a Laboratory Evaluation Officer. A full list of Pennsylvania IMS listed laboratories can be found at on the Interstate Milk Shippers List under Branch 1. The laboratories on this list meet the standards of the Pasteurized Milk Ordinance to test dairy products based on approved lab methods. The applicable 2400 forms that should be used based on the type of testing method can be found under the FDA Forms section of the website.
A full list of Pennsylvania Appendix N Screening only laboratories can be found on the Appendix N Screening Only Dairy Laboratories list.
Approved Lab Procedures in PA
Type | Method | Codes | Procedure Name |
BACTERIA | Agar Plate Count Methods | 2a. | Standard Plate Count (SPC) |
BACTERIA | Agar Plate Count Methods | 2b. | Advanced Instruments Spiral Plate Count (Raw Milk Only) |
BACTERIA | Plate Loop Count Methods (Raw Milk Only) | 3a. | SPC Agar (PLC) |
BACTERIA | Plate Loop Count Methods (Raw Milk Only) | 3b. | 3M PAC (PPLC) |
BACTERIA | Plate Loop Count Methods (Raw Milk Only) | 3c. | 3M RAC (RPLC) |
BACTERIA | Other Total Bacteria Count Methods | 5a. | 3M Petrifilm Aerobic Count (PAC) |
BACTERIA | Other Total Bacteria Count Methods | 5b. | 3M Petrifilm Rapid Aerobic Count (RAC) |
BACTERIA | Other Total Bacteria Count Methods | 5c. | Charm Peel Plate Aerobic Count PPAC |
BACTERIA | Instrument Bacteria Count Methods | 7a. | Foss Bacto Scan FC (BSC) - Raw Milk |
BACTERIA | Instrument Bacteria Count Methods | 7b. | bioMérieux TEMPO Aerobic Count (TAC) |
BACTERIA | Instrument Bacteria Count Methods | 7c. | Bentley BactoCount IBC (BCC) - raw milk only |
BACTERIA | Instrument Bacteria Count Methods | 7d. | Bentley BactoCount IBCm (BCMC) - raw milk only |
BACTERIA | Instrument Bacteria Count Methods | 7e. | Foss BacSomatic - raw milk only |
DRUG TESTS | Detection of Inhibitory Substances | B2 | Charm BsDA |
DRUG TESTS | Detection of Inhibitory Substances | C2 | Charm II Competitive |
DRUG TESTS | Detection of Inhibitory Substances | C3 | Charm II Sequential |
DRUG TESTS | Detection of Inhibitory Substances | C4 | Charm II Quantitative |
DRUG TESTS | Detection of Inhibitory Substances | C10 | Charm II Sulfa Assay |
DRUG TESTS | Detection of Inhibitory Substances | C11 | Charm II Chloramphenicol |
DRUG TESTS | Detection of Inhibitory Substances | C12 | Charm II Tetracycline Assay |
DRUG TESTS | Detection of Inhibitory Substances | C13 | Charm SL Test Beta-lactam |
DRUG TESTS | Detection of Inhibitory Substances | C15 | Charm SL - 3 Test Beta Lactam |
DRUG TESTS | Detection of Inhibitory Substances | C17 | Charm Rosa Tetracycline-SL Test |
DRUG TESTS | Detection of Inhibitory Substances | C18 | Charm Rosa Sulf Test |
DRUG TESTS | Detection of Inhibitory Substances | C19 | Charm Trio Test |
DRUG TESTS | Detection of Inhibitory Substances | C20 | Charm BL30SEC Test |
DRUG TESTS | Detection of Inhibitory Substances | D3 | DelvoTest P 5 Pack |
DRUG TESTS | Detection of Inhibitory Substances | I1 | Idexx New Snap BL |
DRUG TESTS | Detection of Inhibitory Substances | I2 | Idexx- Snap Tetracycline |
SOMATIC CELLS | Direct Somatic Cell Count | 12 | Direct Somatic Cell Count (DMSCC) |
SOMATIC CELLS | Electronic Somatic Cell Count | 16a | Foss 90 |
SOMATIC CELLS | Electronic Somatic Cell Count | 16b. | Foss 250/300/360/400 |
SOMATIC CELLS | Electronic Somatic Cell Count | 16c. | Bentley 150/300/500/FCM/FC |
SOMATIC CELLS | Electronic Somatic Cell Count | 16d. | Foss 5000/FC/7 |
SOMATIC CELLS | Electronic Somatic Cell Count | 16e. | Perkin-Elmer/Delta Instruments MKII/SMART |
SOMATIC CELLS | Electronic Somatic Cell Count | 16f. | Foss Minor |
SOMATIC CELLS | Electronic Somatic Cell Count | 16g. | Foss 7 DC |
SOMATIC CELLS | Electronic Somatic Cell Count | 16h. | Foss BacSomatic |
COLIFORMS | Instrument Coliform Count Methods | 19a. | bioMérieux TEMPO Coliform Count (TCC) |
COLIFORMS | Other Coliform Count Methods | 20a. | 3M Petrifilm Coliform Count (PCC)/HSCC) |
COLIFORMS | Other Coliform Count Methods | 20b. | Charm Peel Plate Coliform Count (PPCC/PPEC/PPCCHV/PPECHV) |
COLIFORMS | Agar Coliform Count Methods | 21a. | Coliform Plate Count (CPC) |
CONTAINERS | Pasteurized milk Containers | 22a. | Rinse Method |
CONTAINERS | Pasteurized milk Containers | 22b. | Swab Method |
CONTAINERS | Other Pasteurized milk Container Methods | 23a. | Disintegration Test |
CONTAINERS | Other Pasteurized milk Container Methods | 23b. | Flat Lid or Pour Contact Tests |
DAIRY WATER | Dairy Water | 24a. | Multiple Tube Fermentation – Most Probable Number (MPN) or Presence-Absence (P-A) |
DAIRY WATER | Dairy Water | 24b. | Membrane Filtration |
DAIRY WATER | Dairy Water | 24c. | Heterotrophic Plate Count (HPC) |
DAIRY WATER | Dairy Water | 24d. | Idexx Colilert |
DAIRY WATER | Dairy Water | 24e. | Idexx Colilert 18 |
DAIRY WATER | Dairy Water | 24f. | Idexx Colisure |
DAIRY WATER | Dairy Water | 24g. | Charm E*colite |
DAIRY WATER | Dairy Water | 24h. | Charm Colitag |
DAIRY WATER | Dairy Water Testing, Other (ex. EPA) | 25 | Dairy Water Testing, Other (ex. EPA) |
PHOSPHATASE | Alkaline Phosphatase Methods | 28a. | Advanced Instruments Flourophos |
PHOSPHATASE | Alkaline Phosphatase Methods | 28b. | Charm Paslite |
PHOSPHATASE | Alkaline Phosphatase Methods | 28c. | Charm Fast Alkaline Phosphatase (FAP) |
PHOSPHATASE | Alkaline Phosphatase Methods | 28d. | Neogen Accupoint Advanced Alkaline Phosphatase Method |
VITAMINS | Vitamin Analysis | 30a. | Vitamin A |
VITAMINS | Vitamin Analysis | 30b. | Vitamin D |
VITAMINS | Vitamin Analysis | 30c. | Vitamin A & D |
Dairy Laboratory Directors
Dairy Laboratory Directors are required for any Industry or commercial laboratory. The requirements for dairy laboratory directors can be found in Ch. 59a.5. Standards for Pennsylvania-approved dairy laboratories, official laboratories and other laboratories; reports of results. The PA Approved Dairy Director Examinations will be distributed electronically on an as needed basis.
The examination is comprised of eight (8) total sections:
1) Sampling, 2) Cultural Procedures, 3) Standard Plate Count [Agar &/or Petrifilm], 4) Coliform Count [Agar &/or Petrifilm], 5) Delvo, 6) Somatic Cell Count [DMSCC & /or ESCC], 7) Appendix N [all assays], 8) Phosphatase test [Charm Paslite, Charm F-AP, &/or Fluorophos]
A twenty dollar ($20.00) fee is assessed to each section of the examination. At minimum, all candidates must take "Sampling" and "Cultural Procedures" and may register for additional sections as desired. The exams are open book, any reference and/or study material may be utilized. However, each candidate must complete the exam independently without assistance from others.
To register, complete the registration form and submit payment in the form of a check made payable to "Commonwealth of Pennsylvania" to the below address:
Pennsylvania Department of Agriculture
Bureau of Food Safety & Laboratory Services
ATTN: Phillip Harchack
2301 North Cameron Street
Harrisburg, PA 17110
Upon successful completion of the Dairy Lab Director exam, a certificate will be issued by the Department.
Appendix N Drug Residue Training Program
This site contains training documents associated with Appendix N Drug Residue testing and QC forms that are required for record keeping. A test will be required for completion of the training program and will be in an online format. A score of 80% is a passing grade. Passing test scores will be maintained as proof of training.
Training of Certified Industry Supervisors (CIS) and Industry Supervisors (IS) that are listed at their testing facility is required according to the current version of FDA Evaluation of Milk Laboratories Manual (EML).
Participation in a training program with a passing test score would be acceptable for the required training of CIS and IS.
The CIS quiz and IS quiz are available on demand at anytime.
This training must be completed every two (2) years during the onsite evaluation. Failure to do so could result in removal as a CIS or IS.
National Milk Drug Residue Database
The National Milk Drug Residue Database (NMDRD) system was implemented in cooperation with the National Conference on Interstate Milk Shipments. The goal is to have a database that consists of testing of milk samples for drug residues by the industry, states, and federal agencies. The data shall include information on the source of samples, when analyses were conducted, numbers of analyses, methods used, and results by drug and methods.
Results shall be submitted monthly to the Department at RA-AGNMDRDREPORTS@pa.gov using the NMDRD Base Reporting Form.
The original PDF form should be submitted to ensure proper data entry. Scanned copies are not acceptable.
The NMDRD Reporting Instructions provides directions on how to fill out this form.
QC Forms
QC (Quality Control) Forms ensures that a laboratory is following the Evaluation of Milk Laboratory Manual, current edition. These forms are necessary to accurately capture required documentation that will be reviewed during the Laboratory Audit conducted by the Department's LEO's to verify accuracy of laboratory procedures.
Applicable QC forms for dairy laboratories can be found in the QC Forms section of the website.
Emergency Lab Reporting Procedures
All positive official monthly and semi-annual test results are required to be reported to the Department of Agriculture immediately, by emailing the BFSLS 472 Emergency Laboratory Report Form, a copy of the positive test results and any supporting documentation applicable (i.e. bill of lading) to RA-AGLABRESULTS@pa.gov.
Screening Facilities – A copy of this report shall accompany the truck and producer samples to the confirmation location, be kept on file at the screening location, and be sent to the Pennsylvania Department of Agriculture with the BFSLS 477 Appendix N Bulk Tanker Positive Drug Residue Test Report immediately upon identifying a positive result.
NCIMS Forms
FORM NCIMS 2400 Cultural Procedures - General Requirements 03/2024
FORM NCIMS 2400a Standard Plate Count and Coliform Plate Count Rev. 03/2024
FORM NCIMS 2400a-1 Spiral Plate Count Rev. 03/2024
FORM NCIMS 2400a-2 Foss BactoScan™ FC Rev. 03/2024
FORM NCIMS 2400a-2 Foss BactoScan FC BIO Companion Protocol Rev. 03/2024
FORM NCIMS 2400a-3 Standard Plate Loop Count Rev. 03/2024
FORM NCIMS 2400a-4 Petrifilm™ Aerobic & Coliform Count Methods Rev. 03/2024
FORM NCIMS 2400a-5 Plate Loop Count - Petrifilm™ Rev. 03/2024
FORM NCIMS 2400a-6 Charm Peel Plate Aerobic and Coliform Count Rev. 03/2024
FORM NCIMS 2400a-7 bioMerieux TEMPO® Aerobic and Coliform Count Rev. 03/2024
FORM NCIMS 2400a-8 Bentley BactoCount IBC Rev. 03/2024
FORM NCIMS 2400a-8 Bentley BactoCount IBC BCIO Companion Protocol Rev. 03/2024
FORM 2400a-9 Bentley BactoCount IBCm BCMIO Companion Protocol Rev. 03/2024
FORM NCIMS 2400a-10 Fossomatic BacSomatic™ Rev. 03/2024
FORM NCIMS 2400b-1 Charm BsDA Rev. 03/2024
FORM NCIMS Delvotest® P 5 Pack 2400b-3 Rev. 03/2024
FORM NCIMS 2400d Direct Microscopic Somatic Cell Count Rev. 03/2024
FORM NCIMS 2400d-1 Delvotest P/SP Mini Rev. 03/2024
FORM NCIMS 2400h FossomaticTM 90 Rev. 03/2024
FORM NCIMS 2400h-1 Fossomatic 250/300/360/400 Rev. 03/2024
FORM NCIMS 2400h-2 Bentley Somacount™ Rev. 03/2024
FORM NCIMS 2400h-3 Fossomatic 5000/FC/7 Rev. 03/2024
FORM NCIMS 2400h-4 Delta - a PerkinElmer Company - SomaScope™ MKII/Smart Rev. 03/2024
FORM NCIMS 2400h-5 Foss Minor Rev. 03/2024
FORM NCIMS 2400h-6 Foss 7 DC Rev. 03/2024
FORM NCIMS 2400i Pasteurized Milk Containers Rev. 03/2024
FORM NCIMS 2400j-1 Advanced Instruments Fluorophos ALP Test Rev. 03/2024
FORM NCIMS 2400j-2 Charm Paslite Alkaline Phosphatase Rev. 03/2024
FORM NCIMS 2400j-3 Charm Fast Alkaline Phosphatase Rev. 03/2024
FORM NCIMS 2400j-4 Neogen AccuPoint Advanced Alkaline Phosphatase Rev. 03/2024
FORM NCIMS 2400k Flat Lid Method Rev. 03/2024
FORM NCIMS 2400l Disintegration Methods Rev. 03/2024
FORM NCIMS 2400m Dairy Waters Rev. 03/2024
FORM NCIMS 2400n Appendix N - General Requirements Rev. 03/2024
FORM NCIMS 2400n-1 Charm SL-SL3-BL30SEC Rev. 03/2024
FORM NCIMS 2400n-2 IDEXX New Snap® Beta-Lactam Test Rev. 03/2024
FORM NCIMS 2400n-4 Charm® II Beta-Lactam Assays Rev. 03/2024
FORM NCIMS 2400n-5 Charm® II Combined Non-Beta-Lactam Assays Rev. 03/2024
FORM NCIMS 2400n-6 Charm FLUSLBL Rev. 03/2024
FORM NCIMS 2400n-7 Neogen BetaStar Advanced for Tetracyclines Rev. 03/2024
FORM NCIMS 2400n-8 Charm® ROSA® TETRACYCLINE-SL Rev. 03/2024
FORM NCIMS 2400n-9 Neogen BetaStar Advanced for Beta-lactams Rev. 03/2024
FORM NCIMS 2400n-10 Charm® ROSA® SULF Rev. 03/2024
FORM NCIMS 2400n-11 Charm® TRIO Test Rev. 03/2024
FORM NCIMS 2400n-12 Idexx SNAP Tetracycline Rev. 03/2024
QC Forms
BFSLS 472 Emergency Lab Report (PDF)
BFSLS 480 Microbological Suitable (MS) Water (PDF)
BFSLS 481 Annual Microscope Calibration (PDF)
BFSLS 485 Daily ph Meter Maintenance (PDF)
BFSLS 485b Daily pH Meter Calibration Log (PDF)
BFSLS 486 Stock Phosphate Buffer (Purchased) (PDF)
BFSLS 486b Stock Phosphate Buffer (Lab Prepared) (PDF)
BFSLS 487 Dilution Water Toxicity Test (PDF)
BFSLS 489 Detergent Residue Test (PDF)
BFSLS 490 Quarterly Percent Weight Loss Records-Agar Method (PDF)
BFSLS 490a Quarterly Percent Weight Loss Records-Petrifilm Method (PDF)
BFSLS 491 Media Information (PDF)
BFSLS 492 Dilution Blank Checks (PDF)
BFSLS 492a Pre-Dispensed Rinse Solution Checks for Gallon Containers (PDF)
BFSLS 492b Pre-Dispensed Rinse Solution Checks for Half Pint-Pint-and Quart Containers (PDF)
BFSLS 492c Pre-Dispensed Rinse Solution Checks for Half Gallon Containers (PDF)
BFSLS 493 Autoclave Sterilization Record (PDF)
BFSLS 494 Hot Air Oven Sterilization Record (PDF)
BFSLS 495 Incubator Temperature Log (PDF)
BFSLS 497 Monthly Analytical Balance Check Records (PDF)
BFSLS 498 MonthlyMilk-Media Electronic Balance Check Records (PDF)
BFSLS 500 Drug Screening Test Log (PDF)
BFSLS 500a Daily Screening Log (PDF )
BFSLS 501a Temperature Record Block Heater (PDF)
BFSLS 501b Temperature Record Freezer (PDF)
BFSLS 501c Temperature Record Refrigerator (PDF)
BFSLS 503 Semi-Annual Pipettor Accuracy Check (PDF)
BFSLS 503b Volume Check Continuous Pipetting Outfit (PDF)
BFSLS 503c Quarterly Dispenser Accuracy Check (PDF)
BFSLS 510 Refrigerator Temperature Log (PDF)
BFSLS 511 Bromthymol Blue Check (PDF)
BFSLS 513 Test Kit Suitability Check Drug Residue Testing (PDF)
BFSLS 513a Positive Control Suitability (PDF)
BFSLS 513b Negative Control Suitability (PDF)
BFSLS 515 Thermometer Accuracy check (PDF)
BFSLS 515a Thermometer Accuracy check (PDF)
BFSLS 527 Microbic Density Records (PDF)
BFSLS 528 Appendix N Training Log (PDF)
BFSLS 528a Appendix N Training Sheet New Analyst (PDF)
BFSLS 530 Semi-Annual DMSCC Syringe Accuracy Check (PDF)
BFSLS 532 Freezer Temperature Log (PDF)
BFSLS 532a Freezer Temperature Log Multi shelves (PDF)
BFSLS 534 Snap Shot Performance Check Set (PDF)
BFSLS 534a Snap Control Checks (PDF)
BFSLS 535 Charm Rosa Reader (Primary Calibration Strips) (PDF)
BFSLS 536a OSSC Work Sheet - Bentley Somacount (PDF)
BFSLS 536b OSSC Work Sheet - Foss (PDF)
BFSLS 544 Lab Director Application (PDF)
BFSLS 547 Comparative Count Work Sheet for RpSM Program (PDF)
BFSLS 547b Comparative Count Work Sheet for less than 3 analsyts (PDF)
BFSLS 548 Autoclave Cycle Timing Records (PDF)
BFSLS531 Advanced Instruments ALP Fluorophos QC Checklist (PDF)